Fig. 10.

Response of U2OS to MDX1338 after p53 silencing. A) Western Blot analysis of p53 showed a marked reduction of protein expression after MDX1338 incubation. p53 levels were not affected by CTRL siRNA. B) CTRL siRNA transfected cells exposed to MDX1338 for 48 h responded by increasing apoptotic fraction, while no differences occurred in p53siRNA transfected cells. C) A slight delay in cell cycle progression was seen in both CTRL siRNA and p53 siRNA transfected cells after MDX1338 treatment.

Actin = reference protein; C= control; CTRLsiRNA= Negative Control siRNA,.