Figure 4.

The anti-cancer effects of the PB nanospheres and the hydrogel against 4T1 mouse breast cancer cells. (A) The fluorescence images of the 4T1 cells after being treated by the PB nanospheres differently, the red color represents the dead cells stained by propidium iodide and the blue color represents the nucleus of all the cells; the white line is the boundary of the 808 nm laser; (B) the fluorescence images of the lower 4T1 cells after being treated by the blank hydrogel and PB hydrogel in the upper transwell, the red color represents the dead cells stained by propidium iodide and the blue color represents the nucleus of all the cells; (C) the survival rates of the 4T1 cells and the L02 cells measured by the MTT test after being treated with the PB nanospheres, ^**P < 0.01. Scale bar: 200 μm.