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. 2019 May 30;9:8059. doi: 10.1038/s41598-019-44592-7

Figure 5.

Figure 5

Isothermal titration calorimetry of Rv0187 with SAM. The SAM binding affinity as measured by ITC experiments in the absence (a) and in the presence of 10 mM magnesium chloride (b) or 10 mM strontium chloride (c). Representative plots of raw heat changes (top) and the normalized data (bottom) are shown. Dissociation constants (Kd) and errors are averages and the standard errors from three independent experiments, respectively.