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Determination of the optimal conditions for recombinant DMP1 expression in E. coli using Western blot analysis probed with anti-histidine conjugated with HRP. The parameters included culturing temperature (28 °C and 37 °C), incubation time (2, 4 and 6 h), and concentration of IPTG (0.2 mM, 0.5 mM and 1 mM) with a negative control (E. coli without pET22b-DMP1).
