Fig. 4.

Purification of the 6xHis-tagged DMP1 fusion protein using Ni-NTA affinity chromatography. The crude extract was subjected to a Ni-NTA column and analysed by SDS-PAGE under reducing conditions, followed by (A) blotting onto a nitrocellulose membrane and probing with HRP-conjugated goat anti-His antiserum or (B) staining with Coomassie brilliant blue. (A) Western blot analysis of purified DMP1 from E. coli probed with anti-histidine conjugated with HRP. (B) SDS-PAGE Lane 1: crude extract; Lane 2: flow through, Lane 3: wash, Lane 4: eluate.