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. 2019 May 23;23:e00348. doi: 10.1016/j.btre.2019.e00348

Table 1.

Primer sequence for gene expression analysis using RT-PCR.

Gene Forward (F) or Reverse (R) Sequences Reference
GAPDH F 5’ CACTGCCAACGTGTCAGTGGTG 3’ NM001289745.2
R 5’ GTAGCCCAGGATGCCCTTGAG 3’
ALP F 5’ CGAGATACAAGCACTCCCACTTC 3’ NM000478.3
R 5’ CTGTTCAGCTCGTACTGCATGTC 3’
CBFA1 F 5’ ATGATGACACTGCCACCTCTGA 3’ NM001024630.3
R 5’ GGCTGGATAGTGCATTCGTG 3’
COL1 F 5’ GTGCTAAAGGTGCCAATGGT 3’ NM000088.3
R 5’ ACCAGGTTCACCGCTGTTAC 3’
OSX F 5’ GCCAGAAGCTGTGAAACCTC 3’ NM001300837.1
R 5’ GCTGCAAGCTCTCCATAACC 3’
OPN F 5’ AGGAGGAGGCAGAGCACA 3’ NM001040060.1
R 5’ CTGGTATGGCACAGGTGATG 3’
WNT3a F 5’ CTGTTGGGCCACAGTATTCC 3’ NM033131.3
R 5’ GGGCATGATCTCCACGTAGT 3’