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. Author manuscript; available in PMC: 2019 May 31.
Published in final edited form as: Arch Toxicol. 2013 Feb 6;87(7):1231–1244. doi: 10.1007/s00204-013-1017-5

Figure 2.

Figure 2.

Effects of in vivo Mn exposure for twenty days on the phosphorylation of MAPK, AKT, CREB and DARPP-32 in the striatum of immature rats. The panels show representative immunoblots and quantification of phosphorylation of p38MAPK (A), AKT (B), DARPP-32-Thr-34 and-Thr-75 (C) and ERK1/2, JNK1/2, CREB and β-Actin (D) in striata of rats treated with saline (control; NaCl 0.9%) or MnCl2 at doses of 5, 10 or 20 mg/kg/day (PN8–27). The structures were analyzed on PN29. The total and phosphorylated form of each protein was detected with specific antibodies and the reaction was developed by chemiluminescence. The phosphorylation level of each protein was determined as the ratio of O.D. of the phosphorylated band/O.D. of the total band, and the data are expressed as percentage of the control (considered as 100%). Values are presented as mean ± S.E.M. Statistical analysis was performed by ANOVA followed by Duncan’s test. n = 12; ** p < 0.01, *** p < 0.001 compared to control.