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. Author manuscript; available in PMC: 2019 May 31.
Published in final edited form as: Arch Toxicol. 2013 Feb 6;87(7):1231–1244. doi: 10.1007/s00204-013-1017-5

Figure 6.

Figure 6.

Effects of in vivo exposure to Mn and/or Trolox™ for twenty days (PN8-PN27) on phosphorylation of p38MAPK (A) and AKT (B) in the striatum of immature rats. The panels show representative immunoblots and quantification of p38MAPK and AKT phosphorylation from rats treated with saline (control; NaCl 0.9%), MnCl2 20 mg/kg (Mn), Trolox™ 1 mg/kg (Tr) or MnCl2 20 mg/kg plus Trolox™ 1 mg/kg (Mn + Tr) for twenty days (PN8–27). The structures were analyzed on PN29. The total and phosphorylated form of each protein was detected with specific antibodies and the reaction was developed by chemiluminescence. The phosphorylation level of each protein was determined as a ratio of O.D. of the phosphorylated band/O.D. of the total band and the data are expressed as percentage of the control (considered as 100%) and the values are presented as mean ± S.E.M. Statistical analysis was performed by ANOVA followed by Duncan’s test. n = 12; * p < 0.05, ** p < 0.01, compared to control; ## p < 0.01 compared to 20 mg Mn/kg group.