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. 2019 May 22;24:101228. doi: 10.1016/j.redox.2019.101228

Fig. 2.

Fig. 2

Thyroid hormone activation via D2 induces a shift from OxPhos to glycolysis.(A, B) pTRE-D2 cells were treated with 2 μg/ml doxycycline for 24 h and 48 h and the rate of OXPHOS and glycolysis was measured with the Seahorse analyzer. OCR was measured continuously throughout the experiment at baseline and in the presence of the indicated drugs. (C) UCP3 mRNA was measured by real time PCR analysis in pTRE-D2 cells treated with doxycycline for 48 h. Cyclophilin A served as internal control. Normalized copies of UCP3 in untreated cells (CTR) were set as 1. (D, E) ATP production was measured in pTRE-D2 cells treated with doxycycline for 48 h by using the ATPlite kit. (F) Expression levels of the myosin heavy chain isoforms (MHC) were measured by real time PCR analysis in pTRE-D2 cells treated with 2 μg/ml doxycycline for 24 h. All the experiments were performed in proliferating cells; the results are shown as means ± SD from at least 3 separate experiments. *P < 0.05, **P < 0.01, ***P < 0.001.