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. 2019 May 22;24:101228. doi: 10.1016/j.redox.2019.101228

Fig. 6.

Fig. 6

SOD2 silencing attenuates TH-mediated myogenic differentiation.(A) mRNA expression of Desmin, Myogenin and MHC were measured in differentiated pTRE-D2 cells treated or not with doxycycline and transfected with SOD2.1 shRNA or CTR shRNA as indicated. (B) Immunofluorescence analysis of Desmin and Myogenin expression of the same cells as in A. Scale bars represent 200 μm and 50 μm, respectively. (C) Levels of total ROS and mitochondrial ROS were measured by FACS analysis of the same cells as in A. (D) D2 up-regulation increases myogenic differentiation and reduces ROS production. SOD2 silencing attenuates the pro-differentiative ability of D2. **P < 0.01, ***P < 0.001.