Fig. 8.

Oxidative modification interferes with laminin-111 self-polymerization. Oxidized and native laminin-111 was dissolved in 50 mM sodium acetate, pH 4 supplemented with 10 mM CaCl₂, and incubated for 60 min to promote polymerization. Panel A) Polymerized laminin-111 was seperated from non-polymerized proteins by centrifugation, and protein content in the 2 fractions was quantified by BCA assay. The percent polymerization was calculated as: $\frac{[Protei{n}_{pellet}]}{[Protei{n}_{pellet}]+[Protei{n}_{supernatant}]}\times 100$. Values are expressed as means ± SEM (n = 3). Panel B) Scanning electron micrographs at 1,000X magnification were recorded for polymerized laminin-111, either left untreated (top image) or exposed to a 500-fold molar excess of ONOOH (bottom image). Scale bar = 100 μm.