Fig. 9.

Nitration reduces cell adhesion to laminin-derived C16 peptide. A) Crystal structure of the N-terminal domain of the laminin-$\gamma 1$ chain shows solvent exposure of Tyr-145 in the C16 sequence (structure from Ref. [62]). The C16 peptide was left untreated (0X) or nitrated with tetranitromethane (TNM), either in 10-fold (10X) or 100-fold (100X) molar excess to the peptide, and used to coat 96-well cell culture plates. HCAECs stained with Calcein-AM were allowed to adhere to the pre-coated 96-well plates for 90 min. After removal of nonadherent cells, the adherent cells were quantified using Calcein-AM fluorescence. Values are expressed as means ± SEM (n = 3). Asterisks indicate significant difference from control samples (ANOVA with Dunnett's correction for multiple testing, ***p < 0.001).