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. 2019 May 16;16:79–93. doi: 10.1016/j.isci.2019.05.021

Figure 3.

Figure 3

Kis Can Promote Transcription via Putative Enhancer Site 16 kb Upstream of EcR-B TSS

(A and B) Representative images of UAS:GFP; GMR46E06-Gal4 expression in larval and pupal brain, respectively. Arrow in (B) denotes location of MB peduncle area.

(C–E) Representative images of (C) control (w1118) and Kis knockdowns (D) (UAS:Kis.RNAi.a) and (E) (UAS:Kis.RNAi.b) in larval MB Kenyon cells stained with α-Gal4 expressed using the GMR46E06-Gal4 enhancer site reporter.

(F) Quantification of fluorescent intensity of α-Gal4 (from left to right, n = 10, 10, 8 MBs).

(G) gal4 mRNA levels analyzed by RT-qPCR (n = 3, 10 heads/biological replicate).

(H–J) ChIP-qPCR analysis of (H) Kis enrichment, (I) H3K36me2, and (J) H4K16ac at B site 2011 from Boulanger et al., (2011), respectively (from left to right, n = 5, 5, 2, 3, 6, 7 biological replicates).

Scale bars: 4 μm in (A), 100 μm in (B), and 20 μm in (C). Statistical significance is represented by * = p < 0.05, ** = p < 0.01, and *** = p < 0.001. Error bars represent the SEM.