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. 2019 May 24;7:113. doi: 10.3389/fbioe.2019.00113

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Copyright © 2019 Fathi-Achachelouei, Knopf-Marques, Ribeiro da Silva, Barthès, Bat, Tezcaner and Vrana.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A-C) Osteogenic differentiation of 3T3 cells under inflammatory state. (A) ALP staining and (B) ALP quantitative assay of 3T3 cells after cultured for 7 and 14 days. (C) mRNA expression of osteogenesis-related genes (ALP, OCN, OPN, COL1, RUNX2) in 3T3 cells cultured for 7 and 14 days. ^*p < 0.05 vs. BGSE (bioactive glass scaffolds extracts) group; ^#p < 0.05 vs. RAW (macrophage-like cell line) group. (D–F) Osteogenic differentiation of 3T3 cells in anti-inflammatory state. (D) ALP staining and (E) ALP quantitative assay of 3T3 cells after being treated with various samples for 7 and 14 days. (F) mRNA expression of osteogenesis-related genes (ALP, OCN, OPN, COL1, RUNX2) of 3T3 cells incubated with various groups for 7 and 14 days. ^*p < 0.05 vs. blank control; ^#p < 0.05 vs. BGSE group. Reproduced from Zhao et al. (2018) with permission from John Wiley and Sons.