Figure 3.

Trib1-deficient Ly6G⁺ eosinophils are a stable population that does not transition to other lineages. (A) Model of fate-tracking EoCre-mediated deletion of Trib1, and hypothesis of a full transition of Ly6G⁺ eosinophils from Trib1^ΔEos BM to neutrophils. (B) Representative plots of BM from Trib1^+/+ or Trib1^ΔEos-YFP mice, gated on live cells. (C) Representative plots of YFP⁺ BM cells gated on live, YFP⁺ cells. (D) Distribution of YFP⁺ cells by SiglecF and Ly6G expression, as a fraction of YFP⁺ BM cells; n = 3 mice per group, representative of 4 experiments. (E) Representative histogram of Ly6G expression on YFP⁺SiglecF⁺CCR3⁺ cells, Trib1^+/+ (blue), Trib1^ΔEos-YFP (red). (F) Frequency of Ly6G expression on YFP⁺SiglecF⁺CCR3⁺ cells; n = 11 mice per group, pooled from 4 experiments. ****P < .0001; unpaired Student t test. Frequencies and error bars are mean ± SEM of live cells. SSC-A, side scatter area.