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. 2019 Mar 27;133(22):2413–2426. doi: 10.1182/blood.2018872218

Figure 7.

Figure 7.

Trib1 regulates C/EPBα levels to control eosinophil identity. (A) C/EBPα immunoblot analysis of sorted neutrophils (CD11b+Ly6G+SiglecF−F4/80−), and CCR3− and CCR3+ eosinophils (CD11b+SiglecF+F4/80+) from the BM of Trib1+/+ and Trib1ΔHSC mice; β-actin is the loading control. Representative of 2 experiments. (B) C/EBPα immunoblot analysis of sorted GFP+ BM cells from mice transplanted with shRNA-transduced BM cells, 9 weeks after transplantation with β-actin as a loading control. shLuc control targets firefly luciferase; shB9 and shB11 target Cebpa. Sorted GFP+ cells from 3 representative mice are shown. Representative of 2 experiments. (C) Percentage of GFP+ cells expressing control or C/EBPα shRNAs B9 or B11 cells expressing SiglecF with surface expression of SiglecF. Representative of 2 experiments. (D) Mean fluorescence intensity (MFI) of Ly6G expression on BM eosinophils (GFP+CD11b+SiglecF+) expressing either control or C/EBPα shRNAs B9 or B11; n = 4-5 mice per group, representative of 2 experiments. **P < .0052, 2-way analysis of variance with multiple comparisons. Frequencies and error bars are mean ± SEM of GFP+ cells.