Figure 1.

Mouse Model of Chronic Kidney Disease. One or two doses of cisplatin (15 mg/kg) were administered intraperitoneally to mice aged 8-12 weeks. Groups were comprised of 3 mice. Blood was sampled by retro-orbital puncture under brief isoflurane anesthesia at 2-week intervals beginning 2 weeks after the first dose to measure serum creatinine. Mice were sacrificed at 0 weeks, 2 weeks after the 1st dose, 4 weeks after the 1st dose, 2 weeks after the second dose (4 weeks after the 1st dose), and 4 weeks after the 2nd dose (6 weeks after the first dose). At the time of sacrifice mice were anesthetized, GFR was measured by inulin clearance and a final blood sample was obtained. The left kidney was surgically removed and processed for mRNA and protein analysis. The animal was then perfused intravascualarly with buffered 4% paraformaldehyde for several minutes and the right kidney was harvested for preparation of frozen sections and MPM analysis.