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Activation of Regulated Necrosis Following Cisplatin Administration. (A) Western blot analysis of p-RIPK1, p-RIPK3, p-MLKL, t-MLKL, TLR2, and TLR4; GAPDH and β-tubulin used as endogenous controls. Protein isolated from whole kidney lysate. (B) Results are mean ± SE (n=3, *: compared with control, p < 0.05) from triplicate blotting. Western Blots showed increased protein expression for p-RIPK1, pRIPK3, and TLR4 following the first and second doses of cisplatin.
