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Activation of Stress Response Following Cisplatin Administration. Western blot analysis of phospho-Thr183/Tyr185-JNK, phospho-Thr202/Tyr204-ERK, and Total ERK; GAPDH was used as an endogenous control. Results are mean ± SE (n=3, *: compared with control, p < 0.05) from triplicate blotting. Western Blots revealed significantly increased protein expression following the first cisplatin dose (pJNK) and second cisplatin dose (pJNK, pERK).
