Effects of LPS and ROT on the expression of circadian clock core genes and proteins. Rats were given LPS (5 mg/kg, i.p. x1), and 7 months later 20 injections of rotenone (0.5 mg/kg, s.c., 5 time/week for 4 weeks). Two weeks after the last ROT treatment, rats were sacrificed and cortex was collected for analysis. A, mRNA expression of circadian clock core genes; B, The expression of BMAL1 protein; C, The expression of CLOCK protein. Values were mean ± SEM (n = 6–9).
*Significantly different from the control group,
#Significantly different from the LPS group, p<0.05.