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Presence of the silCBA genes decreases relative transcript abundance of pcoA and copA. The abundance of pcoA (A) or copA (B) transcript relative to the housekeeping gene rpoD quantified by quantitative RT-PCR from total RNA prepared from mid-log phase cultures in LB broth supplemented with Cu sulfate. Bars indicate mean relative transcript from five biological replicates ± standard deviation. ∗Indicates that groups indicated by lines were significantly different (p < 0.05).
