Table 1.
Bacterial strains and plasmid used in this study.
| Strain or plasmid | Characteristics and functiona | Source or reference |
|---|---|---|
| BACTERIAL STRAINS | ||
| SC19 | S. suis serotype 2, the wide- type (Strepr) | (Li et al., 2009) |
| ΔmnmE | SC19 mnmE::erm (Strepr Ermr) | This study |
| E. coli DH5α | Cloning host for recombinant vector | Trans |
| PLASMID | ||
| pAT18 | With an Ermr gene expressing erythromycin resistance rRNA methylase | (Trieu-Cuot et al., 1991) |
| pET28a | Expression vector; Kanr | Novagen |
| pSET4s | E. coli—S. suis Shuttle vector; Spcr | (Takamatsu et al., 2001) |
| pSET4s-M | Derived from pSET4s for knocking out gene mnmE in SC19; Spcr Ermr | This study |
| pSET2 | E. coli—S. suis Shuttle vector; Spcr | (Takamatsu et al., 2001) |
| pSET2-CM | Derived from pSET2 for functional complementation of ΔmnmE (Spcr) | This study |