Table 2.
Primers used for PCR amplification and detection.
| Primers | Primers sequence (5′-3′) | Amplification for |
|---|---|---|
| Mup-F | AGGTCGACTCTAGAGGATCC TAATTTTTCTGGCGCATTCAGC |
Upstream border of mnmE |
| Mup-R | CTCTTAAGTTTGCTTCTAAGAT ATCTTTCTTTCTAA |
|
| AATTGTCTTATCTATTAGTG | ||
| Mdown-F | TACGGGGAATTTGTATCGA TGAACAAAAGTCATGT |
Downstream border of mnmE |
| AAGAAAACTTGC | ||
| Mdown-R | AAACGACGGCCAGTGAAT TCTCTCTTGACCTGTTTG |
|
| GGTATTATAAA | ||
| Erm-F | AATTTTAGAAAGAAAGATATC TTAGAAGCAAACTTA |
Ermr gene |
| AGAGTGTGTTGA | ||
| Erm-R | TTCTTACATGACTTTTGTTCATCGA TACAAATTCCCCGTAGG |
|
| MnmE-F | CGGGATCCATGACACAC ACATTTGCAGA |
mnmE gene |
| MnmE-R | CGCTCGAGTTAGTGACTGTCC TTTGATTT |
|
| 1453-F | ATGGTTGTATTTATCAAATC TAAAAAAGA |
Upstream gene of mnmE |
| 1453-R | TTAAGCTACAGCAAATAGCTTTTCTT | |
| 1455-F | GTGAAAGGCGGCATTCCC | Downstream gene of mnmE |
| 1455-R | TCAGTCATTTACCCACACCCC | |
| Test-F | GACAAAATAGCTGGTGTAATAAAG | Upstream to downstream regions of mnmE |
| Test-R | CTGTGTATGAAGGAGTTGAGGC | |
| CM-F | GATTCTGCAGTTTCGACGTCCTTTATC | mnmE gene and its |
| CM-R | CTCGGATCCTTATTTTCCAAGACAGA | Promoter |