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. 2019 May 30;20:295. doi: 10.1186/s12859-019-2911-5

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — A graphical representation of the efficiency (E) values across all cycles taken from a 6-step dilution set. Efficiency is calculated using the formula $E = 2^{\frac{{log}_{2} F_{i} - {log}_{2} F_{0}}{i}} - 1$ . The F_i and i values for calculation are taken directly from Additional file 2: Dataset 1, wells A1 through A6. Since F₀ value is unknown, it was selected from the range of theoretically possible F₀ values (covering 0.007–0.0002) and used in the formula