Fig. 7.

Knockdown of H19 reduces the protein level of MET, ZEB1, and COL1A1 in vitro. a Relative expression of lncRNA H19 was determined by real-time PCR analysis. Results are presented as the mean ± SD (**P < 0.01, ***P < 0.001). H19 knockdown was performed in human colorectal cancer cell lines HT-29 and HCT116. b Western blot analysis demonstrated that knockdown of H19 could downregulate the expression of MET, ZEB1, and COL1A1 in both HT-29 and HCT116 cells at the level of protein. WT wild type, NC negative control, si-H19 cell line transfected with lncRNA H19 Smart Silencer