Fig. 3.

NPL4 promotes proliferation of BC cell by regulating cyclin D1 mRNA stability. a, b T24 cells were transfected with NPL4 overexpression vector or si-NPL4. Western blot analysis and qRT-PCR analysis were performed to exam cyclin D1 and CDK4 protein and mRNA levels, respectively. c T24 cells were transfected with si-NPL4 or negative control (si-NC) then exposed to ActD for 2, 4, 6, and 8 h. Cyclin D1 mRNA levels were detected by qRT-PCR. d T24 cells were transfected with pcDNA3.1–NPL4 or empty vector then exposed to ActD for 2, 4, 6, and 8 h. qRT-PCR analysis was used to exam Cyclin D1 mRNA levels. *P < 0.05, **P < 0.01 vs. control group. e T24 cells were treated with the NPL4 inhibitor, disulfiram, and then exposed to actinomycin D with for different lengths of time. Cyclin D1 mRNA levels were detected by qRT-PCR