Fig. 5.

Knockdown of DXO promotes BC cell proliferation by stabilizing cyclin D1 mRNA. a T24 cells were transfected with si-DXO or negative control. Western blot analysis was performed to detect DXO protein level. Right panel shows densitometric analysis. ***P < 0.001 vs. empty vector. b T24 cells were transfected with si-DXO or si-con and then exposed to actinomycin D for 0, 2, 4, and 8 h. Cyclin D1 mRNA expression was detected using qRT-PCR. *P < 0.05 vs. si-con. c T24 cells were prepared as in a, colony formation assays were performed to measure proliferation ability. d qRT-PCR analysis detected DXO expression in BC and normal bladder tissues. *P < 0.01 vs. normal bladder tissue. e The overall survival of the BC patients was analyzed by Kaplan–Meier Upregulation of DXO was correlated with more a favorable overall survival (P = 0.0206)