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. 2019 May 24;10:469. doi: 10.3389/fgene.2019.00469

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Copyright © 2019 Koutsandreas, Ladoukakis, Pilalis, Zarafeta, Kolisis, Skretas and Chatziioannou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biochemical properties of EstDZ4. (A) Effect of pH on EstDZ4 activity. Enzymic activity was measured in the standard reaction, at pH values ranging from 4 to 10, using the indicated buffers. (B) Effect of temperature on EstDZ4 activity. Enzymic activity was measured at temperatures ranging from 40 to 95°C in the standard reaction. (C) Thermostability of EstDZ4 was evaluated by measurements of its esterolytic activity in the standard reaction after exposure to 75, 75, 80, and 85°C for up to 24 h. The reported values correspond to the mean value from three independent experiments performed in triplicate and the error bars to one standard deviation from the mean value.