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. 2018 Mar 28;35(1):51–58. doi: 10.5511/plantbiotechnology.18.0130a

Figure 1. Plant regeneration from hypocotyl explants of T. ciliata and acclimatization. A: Hypocotyl segments were cut from aseptic seedling. a: the upper of hypocotyl; b: the middle of hypocotyl; c: the lower of hypocoty. B: Shoots induction from hypocotyl in T. ciliata on MS media containing 0.5 mg/l 6-BA, 1 mg/l KT and 0.1 mg/l IBA. a: the explant that was cultured for 7 days, callus inducted; b: the explant that was cultured for 10 days, callus inducted continuously; c: the explant that was cultured for 20 days, shoots elongated from the calli; d: the explant that was cultured for 35 days, shoots elongated continuously; C: Shoot multiplication and elongation on MS medium containing 0.3 mg/l 6-BA and 0.2 mg/l NAA after 30 days of growth; D: Roots formed in half-strength MS with 0.1 mg/l NAA, 1.5% sucrose (w/v) and 0.5% agar (w/v). E: Acclimatized plantlet after 20-day transplantation. F: Acclimatized plantlet after two month; G: Acclimatized plantlet after four month.

Figure 1. Plant regeneration from hypocotyl explants of T. ciliata and acclimatization. A: Hypocotyl segments were cut from aseptic seedling. a: the upper of hypocotyl; b: the middle of hypocotyl; c: the lower of hypocoty. B: Shoots induction from hypocotyl in T. ciliata on MS media containing 0.5 mg/l 6-BA, 1 mg/l KT and 0.1 mg/l IBA. a: the explant that was cultured for 7 days, callus inducted; b: the explant that was cultured for 10 days, callus inducted continuously; c: the explant that was cultured for 20 days, shoots elongated from the calli; d: the explant that was cultured for 35 days, shoots elongated continuously; C: Shoot multiplication and elongation on MS medium containing 0.3 mg/l 6-BA and 0.2 mg/l NAA after 30 days of growth; D: Roots formed in half-strength MS with 0.1 mg/l NAA, 1.5% sucrose (w/v) and 0.5% agar (w/v). E: Acclimatized plantlet after 20-day transplantation. F: Acclimatized plantlet after two month; G: Acclimatized plantlet after four month.