Table 2. Effect of different concentrations of cytokinin and auxin on shoot induction in T. ciliata on MS after 35 days of culture.
| No. | Plant growth regulators and concentration | Shoot regeneration frequency (%) | Number of shoots per explant | ||
|---|---|---|---|---|---|
| 6-BA (mg/l) | KT (mg/l) | IBA (mg/l) | |||
| T1 | 0 | 0 | 0 | 0f | 0c |
| T2 | 0.3 | 0.5 | 0.1 | 19.33±5.03e | 2.00±0b |
| T3 | 0.3 | 1 | 0.1 | 26.67±4.16de | 2.67±0.58ab |
| T4 | 0.3 | 1.5 | 0.1 | 42.00±4.00bc | 2.67±1.15ab |
| T5 | 0.5 | 0.5 | 0.1 | 34.67±7.02cd | 2.33±0.58b |
| T6 | 0.5 | 1 | 0.1 | 58.67±5.03a | 4.00±1.00a |
| T7 | 0.5 | 1.5 | 0.1 | 48.67±4.08b | 2.33±0.58b |
| T8 | 1 | 0.5 | 0.1 | 21.33±9.24e | 2.67±1.15ab |
| T9 | 1 | 1 | 0.1 | 42.00±7.21bc | 2.67±0.58ab |
| T10 | 1 | 1.5 | 0.1 | 24.67±2.31e | 2.67±1.15ab |
Values are mean±standard error in triplicate, each with 50 explants. The different letters behind data mean significantly different from each other at p≤0.05 level, according to Duncan’s multiple range test.