Table 3. Effect of aseptic seedling age and position of hypocotyl on adventitious shoot induction from hypocotyl of T. ciliata.

Effect factors		Shoot regeneration frequency (%)	Number of shoots per explant
Seedling age	15-day-old	28.37±7.39b	3.33±0.58b
	20-day-old	43.82±6.64a	4.67±0.58a
	25-day-old	21.49±3.49b	2.67±0.58b
Position	Upper	45.19±2.57	3.67±1.54
	Middle	43.70±10.02	3.33±0.58
	Lower	40.74±20.12	3.33±0.58

Values are mean±standard error in triplicate, each with 50 explants. All the culture media contained 0.5 mg/l 6-BA, 1 mg/l KT and 0.1 mg/l IBA. The different letters behind data mean significantly different from each other at p≤0.05 level, according to Duncan’s multiple range test.