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. 2018 Mar 30;35(1):59–70. doi: 10.5511/plantbiotechnology.18.0202a

Figure 2. Phylogenetic analysis and conserved domains of ammonium transporters (AMTs). A: Unrooted, maximum likelihood-based phylogenetic tree of AMT family proteins generated in MEGA 6.0 software, using ClustalW for the alignment and 1000 bootstrap replicates. AtAMT1;1–1;5 and AtAMT2 from A. thaliana (accessions no. NM_117425.2, NM_105152.3, NM_113336.3, NM_119012, NM_113335, and NM_129385.5); BrAMT1.1–BrAMT1;5, BrAMT2, and BrAMT2like from B. rapa (XM_009126366.2, XM_009113156.1, XM_009104274.2, XM_009131146.2, XM_009137637.1, XM_009145156.2, and XM_009143483.2); OsAMT1;1–1;3, OsAMT2;1–2;3, OsAMT3;1–3;3 from O. sativa (NM_001059815.1, NM_001053990.1, NM_001053991.1, XM_009378133.2, XM_015787532.1, XM_015766098.1, XM_015766529.1, NM_001058371.2, and NM_001053632.1); PtrAMT1;1–1;5, PtrAMT2;1, PtrAMT2;2, PtrAMT3;1, PtrAMT4;1–T4;4 from P. trichocarpa (XM_002314482.2, XM_002325754.1, XM_002311667.2, XM_002303068.1, XM_002301801.1, XM_002314070.2, XM_002309115.1, XM_002323564.1, XM_002300186.2, XM_002302048.2, XM_002324235.2, XM_002306767.2, and XM_002319034.2); EcAmtB from E. coli (NP_286193); BcAMT2 and BcAMT2like from B. campestris are displayed with a black triangle. B: Conserved domains defining the AMT subfamilies generated using WEBLOGO (http://weblogo.berkeley.edu/logo.cgi/).

Figure 2. Phylogenetic analysis and conserved domains of ammonium transporters (AMTs). A: Unrooted, maximum likelihood-based phylogenetic tree of AMT family proteins generated in MEGA 6.0 software, using ClustalW for the alignment and 1000 bootstrap replicates. AtAMT1;1–1;5 and AtAMT2 from A. thaliana (accessions no. NM_117425.2, NM_105152.3, NM_113336.3, NM_119012, NM_113335, and NM_129385.5); BrAMT1.1–BrAMT1;5, BrAMT2, and BrAMT2like from B. rapa (XM_009126366.2, XM_009113156.1, XM_009104274.2, XM_009131146.2, XM_009137637.1, XM_009145156.2, and XM_009143483.2); OsAMT1;1–1;3, OsAMT2;1–2;3, OsAMT3;1–3;3 from O. sativa (NM_001059815.1, NM_001053990.1, NM_001053991.1, XM_009378133.2, XM_015787532.1, XM_015766098.1, XM_015766529.1, NM_001058371.2, and NM_001053632.1); PtrAMT1;1–1;5, PtrAMT2;1, PtrAMT2;2, PtrAMT3;1, PtrAMT4;1–T4;4 from P. trichocarpa (XM_002314482.2, XM_002325754.1, XM_002311667.2, XM_002303068.1, XM_002301801.1, XM_002314070.2, XM_002309115.1, XM_002323564.1, XM_002300186.2, XM_002302048.2, XM_002324235.2, XM_002306767.2, and XM_002319034.2); EcAmtB from E. coli (NP_286193); BcAMT2 and BcAMT2like from B. campestris are displayed with a black triangle. B: Conserved domains defining the AMT subfamilies generated using WEBLOGO (http://weblogo.berkeley.edu/logo.cgi/).