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. 2018 Mar 30;35(1):59–70. doi: 10.5511/plantbiotechnology.18.0202a

Figure 4. Functional complementation of yeast mutant 31019b cells by BcAMT2 and BcAMT2like. pYES2: empty vector was used as the negative control. pYES2-BcAMT2 and pYES2-BcAMT2like: BcAMT2 and BcAMT2like ORFs cloned into pYES2 vector. Yeast cell suspensions were adapted to an optical density at 600 nm of 1.0 (dilution 1). Then, they were serially diluted by factors of 10, and 3 µl of the yeast cell suspensions at each dilution were spotted on yeast nitrogen base medium. Growth was evaluated after three days of incubation at 30°C.

Figure 4. Functional complementation of yeast mutant 31019b cells by BcAMT2 and BcAMT2like. pYES2: empty vector was used as the negative control. pYES2-BcAMT2 and pYES2-BcAMT2like: BcAMT2 and BcAMT2like ORFs cloned into pYES2 vector. Yeast cell suspensions were adapted to an optical density at 600 nm of 1.0 (dilution 1). Then, they were serially diluted by factors of 10, and 3 µl of the yeast cell suspensions at each dilution were spotted on yeast nitrogen base medium. Growth was evaluated after three days of incubation at 30°C.