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. 2018 Mar 30;35(1):59–70. doi: 10.5511/plantbiotechnology.18.0202a

Table 1. Primers used in the experiments.

Primer name Sequence (5′–3′) Usage
AMT2 F: CAGTGTGCTGGAATTCATGGCCGGCGCTTACGATGCGA Cloning BcAMT2 and BcAMT2like into pYES2 vector using EcoRI and XbaI sites
R: TGCGGCCCTCTAGATTATAAAACAATAGTGACACCTC
AMT2like F: CAGTGTGCTGGAATTCATGGCCGGAGCTTACGGTGCA
R: TGCGGCCCTCTAGATTATAAAACAATGGTAACACCTC
pB2 F:CACGGGGGACTCTAGAATGGCCGGCGCTTACGATGCGA Cloning BcAMT2 and BcAMT2like into pBI121 vector using XbaI and SmaI sites
R: TCCTTTACCCATCCCGGGTAAAACAATAGTGACACCTC
pB2like F: CACGGGGGACTCTAGAATGGCCGGAGCTTACGGTGCA
R: TCCTTTACCCATCCCGGGTAAAACAATGGTAACACCTC
qAMT2 F: CTGTCTGGAATGTAGTGTCG qPCR
R: TCTCCATCTCCCCAAAGAGC
qAMT2like F: AGCTTGGGATTGGAGATGACG qPCR
R: ATGCCTTGTGGCATCGAAC
β-actin F: GTGACAATGGAACTGGAATGG qPCR
R: AGACGGAGGATAGCGTGAGG
GAPDH F: CAGGTTTGGAATTGTCGAGG qPCR
R: GAGCTGTGGAAGCACCTTTC