AMT2 |
F: CAGTGTGCTGGAATTCATGGCCGGCGCTTACGATGCGA |
Cloning BcAMT2 and BcAMT2like into pYES2 vector using EcoRI and XbaI sites |
R: TGCGGCCCTCTAGATTATAAAACAATAGTGACACCTC |
AMT2like |
F: CAGTGTGCTGGAATTCATGGCCGGAGCTTACGGTGCA |
R: TGCGGCCCTCTAGATTATAAAACAATGGTAACACCTC |
pB2 |
F:CACGGGGGACTCTAGAATGGCCGGCGCTTACGATGCGA |
Cloning BcAMT2 and BcAMT2like into pBI121 vector using XbaI and SmaI sites |
R: TCCTTTACCCATCCCGGGTAAAACAATAGTGACACCTC |
pB2like |
F: CACGGGGGACTCTAGAATGGCCGGAGCTTACGGTGCA |
R: TCCTTTACCCATCCCGGGTAAAACAATGGTAACACCTC |
qAMT2 |
F: CTGTCTGGAATGTAGTGTCG |
qPCR |
R: TCTCCATCTCCCCAAAGAGC |
qAMT2like |
F: AGCTTGGGATTGGAGATGACG |
qPCR |
R: ATGCCTTGTGGCATCGAAC |
β-actin |
F: GTGACAATGGAACTGGAATGG |
qPCR |
R: AGACGGAGGATAGCGTGAGG |
GAPDH |
F: CAGGTTTGGAATTGTCGAGG |
qPCR |
R: GAGCTGTGGAAGCACCTTTC |