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. 2019 Jun;60(6):687–694. doi: 10.1165/rcmb.2018-0179OC

Figure 3.

Figure 3.

GABAα and GABAβ signaling has no effect on baseline and IL-13–induced differentiation of air–liquid interface (hALI) cultures of normal human bronchial epithelial (NHBE) cells. (A) GABAβ receptor (GABBR2) expression in ALI cultures at Day 14 of differentiation by double staining for GABBR2 and MUC5AC. Nuclei were stained by DAPI. Arrows mark GABBR2 expression in MUC5AC+ goblet cells and other epithelial cell types. Scale bar: 50 μm. (B) Scheme of the treatment with GABA receptor blockers in control and IL-13–stimulated ALI cultures. IL-13 (10 ng/ml) stimulation started at Day 0. Treatment with single and combined GABAα receptor blocker (50 μM picrotoxin) and GABAβ receptor blocker (1 μM CGP55845) started at Day 14. (C and D) Cultures were analyzed at Day 21 for the gene expression of a ciliated cell marker (FOXJ1) (C) and a club cell marker (CC10) (D). Data represent mean ± SEM of three independent experiments of NHBE cells from each donor and a total of three donors for each condition. ***P < 0.001. n.s. = not significant.