Figure 3.

Overexpression of SDC2 enhances MMP9 expression and A549 cell invasion. Cells were lentivirally transfected with empty vector (EV) or pLenti-SDC2-GFP (SDC2-GFP). (A) Transfection efficiency was confirmed by flow cytometry to detect GFP-positive cells. (B and C) Transfected cells were lysed and MMP9 expression and cell invasion were determined by Western blot or a Matrigel invasion assay, respectively. The data are presented as mean ± SEM, n = 3/group, with testing by Student’s unpaired t test (*P < 0.05, significant comparisons vs. EV).