Figure 6.

Silencing of SDC2 inhibits nuclear accumulation of NF-κB subunit p65 and its binding to the MMP9 promoter in A549 cells. Cells were lentivirally transfected with shSDC2 with or without Synt-1–DDK. (A) Cell lysates underwent nuclear/cytosol fractionation to measure p65 localization. (B) A chromatin immunoprecipitation (ChIP) assay was used to measure p65 binding to the MMP9 promoter. (C and D) Cells were transiently transfected with scr or sip65 RNAs for 24 hours before being collected to measure MMP9 expression or for the Matrigel invasion assay. The data are presented as mean ± SEM, n = 3/group, with testing by Student’s unpaired t test (*P < 0.05, significant comparisons vs. EV or Scr).