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. 2019 May 24;10:1128. doi: 10.3389/fimmu.2019.01128

Figure 2.

Figure 2

Time course of pemphigus IgG-mediated EGFR and ERK activation. Confluent HaCaT cell monolayers were incubated with different IgG fractions for 5 min, 30 min as well as 60 min to evaluate activation due to phosphorylation of (A) EGFR and (B) ERK in immunoblots. EGFR was activated after 30 min by IgG fractions containing antibodies against Dsg3 (n ≥ 7, *p ≤ 0.05, one-way ANOVA, normalized to respective c-IgG). ERK was activated only when antibodies targeting Dsg1 were present (n ≥ 7, *p ≤ 0.05, one-way ANOVA, normalized to respective c-IgG).