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. 2019 May 24;10:1128. doi: 10.3389/fimmu.2019.01128

Figure 4.

Figure 4

EGFR activation reduces binding frequency of Dsg3 interactions on living HaCaT keratinocytes. (A) Atomic force microscopy (AFM) adhesion measurements on cell borders of living HaCaT keratinocytes using a Dsg3 Fc-functionalized tip and 1 h incubation of EGF with representative force maps. A reduction in binding frequency is observable in a Src-dependent manner, (n = 3 with two separate cell borders per experiment, one-way ANOVA, *p ≤ 0.05) whereas (B) binding forces remained unaffected. (C) Cell-free AFM measurements on Dsg3 Fc-functionalized mica sheets prove that reduction in binding frequency is not induced by direct inhibition (n = 3, t-test, *p ≤ 0.05) (D) Binding frequency was reduced in HaCaT cells treated for 1 h with mc-PV2-IgG independently of Src (n ≥ 3, with two separate cell borders per experiment, one-way ANOVA, *p ≤ 0.05).