Analysis of interaction of the (β15–66)2
Lys-Arg-mutants with the fibrin-binding VLDLR(1–8) fragment by SPR. The
(β15–66)2-R42R44K47
mutant at increasing concentrations, 0.1, 0.25, 0.5, 1.0, 2.5, 5, 7.5, and 10
μM (A), or the
(β15–66)2-K53K54R57K58
mutant at 0.25, 0.5, 1.0, 2.5, 5, 7.5, 10, 15, and 20 μM (B) was added to
the immobilized VLDLR(1–8) fragment and its association/dissociation was
monitored in real time while registering the resonance signal (response) using
BIAcore biosensor. The dotted curves in both panels, which essentially coincide
with the experimental curves, represent the best fit of the binding data using
global fitting analysis (see Experimental Procedures). The
Kd values determined from SPR binding data are
presented in Table 3.