Table 2.
β N-fragments | Kd (nM)a | |
---|---|---|
ELISA | SPR | |
(β 15–66)2 fragment | 3.6 ± 0.8 | 3.1 ± 0.2 |
β 15–64 fragment | n.d.b | 6.1 ± 1.6 μ M |
(β 15–44)2 fragment | n.d.b | 67 ± 9 μ M |
Ionic Strength Dependence | ||
(β 15–66)2 + 0.15 M NaCl | 3.0 ± 0.1 | 3.1 ± 0.2 |
(β 15–66)2 + 0.2 M NaCl | 6.0 ± 0.3 | 5.8 ± 0.2 |
(β 15–66)2 + 0.25 M NaCl | 13.5 ± 1.3 | 11.1 ± 0.1 |
(β 15–66)2 + 0.3 M NaCl | 34.1 ± 1.9 | 20.5 ± 2.7 |
All Kd values are in nM except those for the β15–64 and (β15–44)2 fragment, which are presented in μM.
Not detected due to low binding signal.