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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Bioorg Chem. 2019 Feb 13;85:585–599. doi: 10.1016/j.bioorg.2019.02.027

Table 2.

DNA binding parameters for complexes 5c, 5d and 5f.

Compounds Binding constant Ka (M−1)a nb ΔTmc (°C) Hoechst DC50 (μM)d DAPI DC50 (μM)d
5c 0.39 × 105 ± 5291 25.8 ± 0.12 2.18 9.1 ± 2.5 6.2 ± 1.6
5d 0.10 × 105 ± 623.0 30.8 ± 0.08 1.93 19.1 ± 3.7 17.5 ± 5.1
5f 0.30 × 105 ± 8243 25.9 ± 0.25 1.92 33.7 ± 5.1 15.3 ± 8
a

DNA binding constant by UV–vis spectroscopy method.

b

[Polynucleotide Phosphate]/([Bound compound]) Calculated from the UV–Vis titrations of compounds with ds-Polynucleotides.

c

Change in the melting temperature of CT-DNA.

d

The concentration of each inhibitor needed to displace 50% DAPI or Hoechst 33258 from the DNA minor groove at saturating binding levels.