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. 2019 May 24;10:562. doi: 10.3389/fphys.2019.00562

Figure 2.

Figure 2

Effects of nMitoQ treatment on placental and fetal hypoxia. Expression of HIF1-α protein, a marker for tissue hypoxia in placentae obtained from male (A) and female (E) fetuses on GD21. Oxygenation levels as assessed by pimonidazole staining in the placentae of male (B) and female (F), the cardiac tissues of male (C) and female (G) and hepatic tissues of male (D) and female (H) fetuses on GD21. Data are represented as mean ± SEM. a.u.: arbitrary units. All groups were compared using a two-way ANOVA followed by Sidak’s post hoc test (n = 5–6/group). *p < 0.05, **p < 0.01 group effect of prenatal hypoxia and nMitoQ treatment; #p < 0.05, ##p < 0.01 compared to normoxia-saline; †p < 0.05, ††p < 0.01 compared to hypoxia-saline group.