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. 2019 May 22;13:497. doi: 10.3389/fnins.2019.00497

FIGURE 5.

FIGURE 5

Light-induced retinal inflammation (2). (A) Immunohistochemistry was performed on the retinas of blue- (b) and yellow-exposed (y) mice either immediately after illumination (nRec) or in 3 days of recovery (Rec). Results of anti-Iba1 staining is presented. Magnification is 20x, scale bar corresponds to 100 μm. (B–D) Results of RT-qPCR analysis on the retinas: mRNA expression of GFAP (B), ATF3 (C) and Iba1 (D). Statistical significance: - GFAP: blue no recovery vs. recovery – q = 0.0101, p = 0.0096; recovery blue vs. yellow – q = 0.0074, p = 0.0070; - ATF3: blue no recovery vs. recovery – q = 0.0230, p = 0.0219; no recovery blue vs. yellow – q = 0.0057, p = 0.0054; - Iba1: blue no recovery vs. recovery – q = 0.0118, p = 0.0113; yellow no recovery vs. recovery – q = 0.0428, p = 0.0814. Blue and yellow bars correspond to blue and yellow exposures, respectively; clear and hatched bars correspond to the time points of mice dissection, either directly after illumination (no recovery) or in 3 days of recovery (recovery), respectively. All the data are presented as mean ± SEM. Differences were considered significant when p < 0.05 (∗/∧), p < 0.01 (∗∗/∧∧), p < 0.001 (∗∗∗/∧∧∧) or p < 0.0001 (∗∗∗∗/∧∧∧∧). Stars correspond to comparisons between blue-illuminated and yellow-illuminated mice, within one recovery or non-recovery group. Carets correspond to comparison of mice assessed directly after illumination to the ones assessed after 3 days of recovery, within the same spectra. Red color means increase and blue color decrease in values.