Table 2. Twelve microsatellite loci were developed for SSR markers and their amplification in the three species tested (Waikham et al., 2018).

Primers	SSR motif	Primer sequence (5′–3′)	Amplification size range (bp)
			L. polystachyus	C.tribuloides	C.calathiformis
CT097	(ATT)n	F: HEX-CGACTTTGGGAAGGAAATAAAGG	140–152	139–175	126–144
		R: TGGACTTCAACTTGCCATAGTG
CT110	(TGT)n	F: TAMRA-TTCTTCAGTTAGCCACATCG	172–184	169–205	173–179
		R: CGCTAAGTCCATACATACAACAG
CT113	(TTC)n	F: HEX-CCACTCGTAGCAGCCAATAATA	114–195	144–171	115–175
		R: CCACTCGTAGCAGCCAATAATA
CT127	(AGA)n	F: TAMRA-CCCAGAAAACGTATGATCTTTG	191–254	207–246	172–250
		R: CCATGCAACACTACCTCGTC
CT128	(TCA)n	F: TAMRA-CCCTTGGCAGACAAACTAGATA	168–186	160–244	172–193
		R: GGCGCAACAACATATGAAGAAT
CT132	(AT)n(AAG)n	F: TAMRA-TGACCCGAGCATGGTTTAT	158–203	126–174	178–208
		R: GGACGTTAGGCCTGTACATT
CT135	(TGA)n(GAA)n	F: FAM-GCCTAGCTTATGGAGTGGTT	116–146	124–160	123–165
		R: GTCTTTGTGCAAGTGCTC
CT149	(TCT)n	F: FAM-GCGCGTGACTTAGGCTCTTCAC	142–166	144–150	140–161
		R: CTTCTCTGTTGGCATTTCTTGC
CT159	(TCT)n	F: FAM-ATCCATGTCCACTTCTTCAA	132–159	130–193	133–148
		R: CGTTTCCAAAACGAAGAAC
CT161	(CAC)n	F: FAM-AACGATACTAGCGACCTTGA	145–166	136–166	139–166
		R: GCGAAAAACGCTCTCCAAC
CT164	(CAC)n	F: HEX-ACAACACACCTAACATCACAAC	146–164	129–174	132–159
		R: GAATGTTGCTCAGCGAAG
CT165	(CTT)n	F: HEX-AGCGCCTTCTTAATAGAACC	153–186	112–149	119–161
		R: TGGTGACCATTACTTGTTGA