IFT122 is required for optic cup and lens formation and for specification of the RPE and NR. (A) 3-D reconstruction of wild-type and Ift122 mutant eyes at E10.5. Compass abbreviations: A, anterior; Po, posterior; Di, distal; Pr, proximal; V, ventral; D, dorsal. (B) Sections through E9.5 (20–22 somite stage) wild-type and Ift122 mutant optic vesicles stained with markers for cell fates. Note the dorsal expansion of PAX2 and the loss of MITF and CHX10 expression in the Ift122 mutant. (C) Sections through E10.5 (36–40 somite stage) wild type and Ift122 mutant eyes stained with markers for cell fates. Note the loss of MITF and CHX10 expression, reduction of OTX2+ cells, and the distal expansion of SOX1 expression in the Ift122 mutant.