Table 1.
Characteristic of Conserved Signature Indels specific to the class Thermoleophilia or its associated taxa.
| Protein name | GI no.a | Figure number | Indel size | Indel positionb | Specificity |
|---|---|---|---|---|---|
| Quinolinate synthase NadA | 1225101978 | Figure 2 | 4aa insc | 138–180 | All Thermoleophilia |
| 30S ribosomal protein S10 | 1093219170 | Supplementary Figure S2 | 1aa ins | 72–105 | All Thermoleophilia |
| Glutamate-1-semialdehyde-2,1-aminomutase | 1225102988 | Supplementary Figure S3 | 2aa del | 172–209 | All Thermoleophilia |
| D-tyrosyl-tRNA(Tyr) deacylase | 1225105696 | Supplementary Figure S4 | 6aa del | 100–135 | All Thermoleophilia |
| Vitamin B12-dependent ribonucleotide reductase | 1225104123 | Supplementary Figure S5 | 1aa ins | 746–793 | All Thermoleophilia |
| DNA-directed RNA polymerase subunit beta | 1225103324 | Supplementary Figure S6 | 2aa ins | 215–256 | All Thermoleophilia |
| PspA/IM30 family protein | 654611971 | Supplementary Figure S7 | 3aa del | 184–227 | All Thermoleophilia |
| Glutamine-hydrolyzing GMP synthase | 1225105599 | Supplementary Figure S8 | 1aa ins | 406–450 | All Thermoleophilia |
| Elongation factor P | 1225104642 | Supplementary Figure S9 | 1aa ins | 127–176 | All Thermoleophilia |
| Replicative DNA helicase | 1225103017 | Supplementary Figure S10 | 2aa ins | 15–55 | All Thermoleophilia |
| Phenylalanine–tRNA ligase subunit alpha | 654610443 | Supplementary Figure S11 | 2–10aa ins | 244–285 | All Thermoleophilia |
| DNA polymerase III alpha subunit | 1225105080 | Supplementary Figure S12 | 1aa ins | 84–128 | All Thermoleophilia |
| Arginine–tRNA ligase | 1225101858 | Figure 3 | 7aa ins | 314–367 | Thermoleophiliaceae |
| LytR family transcriptional regulator | 1225102507 | Supplementary Figure S13 | 2aa ins | 155–190 | Thermoleophiliaceae |
| DNA gyrase subunit A | 1225102941 | Supplementary Figure S14 | 8aa ins | 250–298 | Thermoleophiliaceae |
| Chaperonin GroEL | 1225103134 | Supplementary Figure S15 | 3aa ins | 459–497 | Thermoleophiliaceae |
| Short chain dehydrogenase | 1225103641 | Supplementary Figure S16 | 2aa ins | 222–264 | Thermoleophiliaceae |
| Type II secretion system F family protein | 1225104607 | Supplementary Figure S17 | 1aa ins | 299–342 | Thermoleophiliaceae |
| Leucyl-tRNA synthetase | 1093217654 | Supplementary Figure S18 | 1aa ins | 429–469 | Thermoleophiliaceae |
| NADH-quinone oxidoreductase subunit B | 551309834 | Figure 4 | 1aa del | 137–181 | Conexibacteraceae, Solirubrobacteraceae, Patulibacteraceae |
| 4-hydroxy-3-methylbut-2-enyl diphosphate reductase | 739551922 | Supplementary Figure S19 | 1aa ins | 44–91 | Conexibacteraceae, Solirubrobacteraceae, Patulibacteraceae |
| Pyruvate kinase | 652636441 | Supplementary Figure S20 | 5aa del | 189–227 | Conexibacteraceae, Solirubrobacteraceae, Patulibacteraceae |
| tRNA guanosine (34) transglycosylase Tgt | 654594575 | Supplementary Figure S21 | 1aa ins | 312–357 | Conexibacteraceae, Solirubrobacteraceae, Patulibacteraceae |
| Excinuclease ABC subunit UvrB | 654612298 | Supplementary Figure S22 | 1aa ins | 215–263 | Conexibacteraceae, Solirubrobacteraceae, Patulibacteraceae |
| Transcription antitermination factor NusB | 494847549 | Supplementary Figure S23 | 6aa ins | 62–102 | Conexibacteraceae, Solirubrobacteraceae, Patulibacteraceae |
| Thioredoxin-disulfide reductase | 916615184 | Figure 5 | 1aa ins | 40–82 | Conexibacteraceae |
| Trigger factor | 917589205 | Supplementary Figure S24 | 5aa ins | 169–217 | Conexibacteraceae |
| Supplementary Figure S25 | 1aa ins | 215–255 | Conexibacteraceae | ||
| Glutamate-5-semialdehyde dehydrogenase | 652642436 | Supplementary Figure S26 | 5aa del | 150–196 | Conexibacteraceae |
| Glutamine amidotransferase | 654598081 | Figure 6 | 3aa ins | 170–211 | Solirubrobacteraceae |
| 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit CofH | 654594367 | Supplementary Figure S27 | 4aa del | 152–192 | Solirubrobacteraceae |
| methionine–tRNA ligase | 654600348 | Supplementary Figure S28 | 5aa ins | 267–310 | Solirubrobacteraceae |
| Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC | 654597239 | Supplementary Figure S29 | 1aa ins | 20–65 | Solirubrobacteraceae |
| CTP synthase | 921290543 | Supplementary Figure S30 | 2aa ins | 264–308 | Solirubrobacteraceae |
| DNA-directed RNA polymerase subunit beta’ | 494853285 | Figure 7 | 8aa ins | 376–420 | Patulibacteraceae |
| SDR family NAD(P)-dependent oxidoreductase | 494848053 | Supplementary Figure S31 | 2aa ins | 149–198 | Patulibacteraceae |
| Dihydrolipoyl dehydrogenase | 551307243 | Supplementary Figure S32 | 1aa del | 355–396 | Patulibacteraceae |
| Methylmalonyl-CoA epimerase | 551310266 | Supplementary Figure S33 | 2aa ins | 1–48 | Patulibacteraceae |
| Acetyl-CoA carboxylase biotin carboxylase subunit | 551309981 | Supplementary Figure S34 | 2aa ins | 224–268 | Patulibacteraceae |
| GTPase HflX | 1225104795 | Supplementary Figure S35 | 1aa ins | 282–322 | Patulibacteraceae |
| 1-deoxy-D-xylulose-5-phosphate reductoisomerase | 551310630 | Supplementary Figure S36 | 6–8aa ins | 146–188 | Patulibacteraceae |
| Tryptophan–tRNA ligase | 494851195 | Supplementary Figure S37 | 4–12aa ins | 152–191 | Patulibacteraceae |
| Endopeptidase La | 551309049 | Supplementary Figure S38 | 1aa ins | 228–266 | Patulibacteraceae |
| 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit CofH | 494847285 | Supplementary Figure S39 | 4aa ins | 481–522 | Patulibacteraceae |
| NADH-quinone oxidoreductase subunit I | 1113228917 | Supplementary Figure S40 | 1aa ins | 72–125 | New cluster |
| Adenylosuccinate synthase | 1113229450 | Supplementary Figure S41 | 17–23aa ins | 154–204 | S.67-14 and S.70-9d |
| GTPase Era | 1113226493 | Supplementary Figure S42 | 1–2aa ins | 38–88 | S.67-14 and S.70-9 |
| Heme-copper oxidase subunit III | 1113215223 | Supplementary Figure S43 | 1–4aa ins | 121–167 | S.67-14 and S.70-9 |
aThe GI number represents the GenBank identification number of the protein sequence from one Thermoleophilia species that contain the specific CSI. bThe indel region indicates the region of the protein where the described CSI is present. cins, insertion; del, deletion. dS.67-14 and S.70-9 are abbreviations for MAG “Solirubrobacterales bacterium 67-14” and “Solirubrobacterales bacterium 70-9.”