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. 2019 May 24;10:473. doi: 10.3389/fgene.2019.00473

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Copyright © 2019 Zhao, Zhang, Qin, Nikolay, He, Spahn and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of protein abundances obtained by different label-free quantification methods. (A) Quantified protein numbers in two biological replications of HRM-MS method of two strains. (B,C) The quantification reproducibility of HRM-MS method in relaxed and stringent criteria of two E. coli strains, respectively. R is the Pearson correlation coefficient. The protein abundance range was divided into low (<2.0 log scale), mid (2.0–3.5 log scale) and high (>3.5 log scale) sections. r_low, r_mid and r_high are the Pearson correlation of the proteins in these sections. (D,E) The reproducibility of DDA MS experiment of the two strains. Proteins were quantified using iBAQ method. (F) The peptide coverage of the HRM-MS-identified proteins in two E. coli strains, respectively.