FIGURE 5.

Examples of “Complex” and “Pathway” operons. (A) The gene expression at RNA and protein levels in E. coli K-12 MG1655 strain. The detailed expression level are marked on the data points. <1 means the expression level is too low to be confidently quantified. (B) Illustrations of the “branch-free” and “branched” pathways. E1 and E2 represent the quantified proteins in the same operon. (C) All nine operons which encodes enzymes that exist in branch-free pathways. Their RNA abundance, protein abundance and K_M values were plotted. K_M values are plotted in orange bars. Detailed K_M values are listed in Table 1. Detailed pathways are illustrated in Supplementary Figure S6.