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. Author manuscript; available in PMC: 2019 May 31.
Published in final edited form as: Cell Rep. 2019 May 14;27(7):1960–1966.e6. doi: 10.1016/j.celrep.2019.04.067

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse monoclonal anti-SV40 VP1 (PAB597) lab generated, cross-reacts with JCPyV VP1 N/A
Rabbit polyclonal anti-5-HT2AR LS Biosciences cat#LS-A1106; RRID: AB_10645379
Rabbit polyclonal anti-5-HT2BR Novus Biologicals cat#NLS1111; RRID: RRID:AB_343312
Rabbit polyclonal anti-5-HT2CR LS Biosciences cat#LS-A1119; RRID: AB_592691
Rabbit polyclonal anti-transferrin receptor Novus Biologicals cat#NB100–92243; RRID: AB_1216384
Rabbit monoclonal anti-β-arrestin1/2 Cell signaling Technologies Cat#4674; RRID: AB_10547883
mouse monoclonal anti-GAPDH Sigma-Aldrich Cat#G8795 RRID:AB_1078991
Mouse monoclonal anti-pan Cadherin Abcam Cat#ab6528; RRID:AB_305544
Rabbit polyclonal anti-6X His tag Abcam Cat#ab9108; RRID:AB_307016
Virus Strains
JCPyV Vacante et al., 1989 N/A
SV40 Norkin and Ouellette, 1976 N/A
Chemicals, Peptides, and Recombinant Proteins
Ritanserin Tocris Bioscience Cat#1955
Hygromycin B ThermoFisher Scientific Cat#10687010
Geneticin (G418) ThermoFisher Scientific Cat#10131035
Puromycin ThermoFisher Scientific Cat#A1113803
Neuraminidase type II Sigma Cat#N6514
Critical Commercial Assays
QuikChange II Site directed mutagenesis kit Agilent Cat#200555
QuikChange II XL Site Directed Mutagenesis Kit Agilent Cat#200521
Q5 Site-Directed Mutagenesis Kit NEB Cat#E0554S
BioLuxCypridina Luciferase Assay kit NEB Cat#E3309L
Fugene HD Promega Cat#E2311
LipofectamineRNAiMAX ThermoFisher Scientific Cat#13778075
Alexa Fluor 488 NHS Ester (Succinimidyl Ester) ThermoFisher Scientific Cat#A200000
Lipofectamine 3000 ThermoFisher Scientific Cat#L3000008
Experimental Models: Cell Lines
SVG-A cells (human fetal glial cells) Major et al., 1985 N/A
HEK293A cells ATCC Cat#CRL-1573; RRID:CVCL_0045
HEK293T cells ATCC Cat#CRL-11268; RRID:CVCL_1926
Lenti-X HEK293T cells Takara cat#632180
CV-1 (African green monkey) ATCC Cat#CCL-70; RRID:CVCL_0229
Oligonucleotides
SignalSilence β-arrestin1 siRNA Cell Signaling Technologies Cat#6218
SignalSilence Control siRNA Cell Signaling Technologies Cat#6568
Mutagenesis primers see tables This paper N/A
Recombinant DNA
lentiCRISPRv2 plasmid-puromycin Addgene Cat#52961; RRID: Addgene_52961
lentiCRISPRv2 plasmid-neomycin This paper N/A
lentiCRISPRv2 plasmid-hygromycin This paper N/A
pCMV-dR8.2 Addgene Cat#8455; RRID: Addgene_8455
pCMV-VSV-G Addgene Cat#8454; RRID: Addgene_8454
5-HT2AR-pcDNA 3.1 UMR cDNA resource center, University of Missouri-Rolla Cat#HTR02A0001
5-HT2BR-pcDNA 3.1 UMR cDNA resource center, University of Missouri-Rolla Cat#HTR02B0000
5-HT2CR-pcDNA 3.1 UMR cDNA resource center, University of Missouri-Rolla Cat#HTR02C0000
5-HT2AR-YFP Assetta et al., 2013; Maginnis et al., 2010 N/A
5-HT2BR-YFP Assetta et al., 2013; Maginnis et al., 2010 N/A
5-HT2CR-YFP Assetta et al., 2013; Maginnis et al., 2010 N/A
5-HT2AR-YFP-P180A This paper N/A
5-HT2BR-YFP-P160A This paper N/A
5-HT2CR-YFP-P159A This paper N/A
5-HT2AR-pcDNA 3.1-trunc before DRY-His This paper N/A
5-HT2AR-pcDNA 3.1-trunc after DRY-His This paper N/A
5-HT2BR-pcDNA 3.1-trunc before DRY-His This paper N/A
5-HT2BR-pcDNA 3.1-trunc after DRY-His This paper N/A
5-HT2CR-pcDNA 3.1-trunc before DRY-His This paper N/A
5-HT2CR-pcDNA 3.1-trunc after DRY-His This paper N/A
5-HT2AR-His-pcDNA 3.1 This paper N/A
5-HT2BR-His-pcDNA 3.1 This paper N/A
5-HT2CR-His-pcDNA 3.1 This paper N/A
VP1-pwP vector Gee et al., 2013 N/A
VP2-ph2p vector Gee et al., 2013 N/A
VP3-ph2p vector Gee et al., 2013 N/A
phSv40-Cluc Gee et al., 2013 N/A
Software and Algorithms
Blobfinder Center for Image Analysis (Uppsala University) http://www.cb.uu.se/amin/BlobFinder/
Fiji Schindelin et al., 2012 https://fiji.sc/
CRISPR design Zhang Lab http://zlab.bio/guide-design-resources
QuikChange primer design tool Agilent https://www.chem.agilent.com/store/primerDesignProgram.jsp
NEBaseChanger tool NEB http://nebasechanger.neb.com/